8JBC

Crystal Structure of the Csm6 K137A mutant from Thermus thermophilus HB8 in its apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2022-08-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978
Spacegroup name	P 21 21 2
Unit cell lengths	94.890, 205.570, 59.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.640 - 2.410
R-factor	0.2242
Rwork	0.222
R-free	0.26930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.112
Data reduction software	HKL-2000
Data scaling software	HKL-2000 (1.20.1_4487)
Phasing software	MOLREP
Refinement software	REFMAC (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.520	2.470
High resolution limit [Å]	2.410	2.410
Number of reflections	45579	4483
<I/σ(I)>	15
Completeness [%]	99.9	99.93
Redundancy	12.9
CC(1/2)	0.999	0.676

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	0.01 M Magnesium chloride hexahydrate, 0.1M HEPES sodium pH 7.0, 15% w/v Polyethylene glycol 3350