8JAH

Crystal structure of human CLEC12A C-type lectin domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	P 61 2 2
Unit cell lengths	50.012, 50.012, 367.627
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.010 - 2.580
R-factor	0.2076
Rwork	0.205
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.587
Data scaling software	XDS
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.010	2.670
High resolution limit [Å]	2.580	2.580
Rmerge	0.206	0.953
Number of reflections	8840	751
<I/σ(I)>	13.357	1.462
Completeness [%]	92.4
Redundancy	13.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	20% (w/v) PEG 3350, 0.2 M Potassium sulfate