8JAE

Crystal structure of E33A mutant human galectin-10 produced by cell-free protein synthesis in complex with melezitose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 65 2 2
Unit cell lengths	49.160, 49.160, 262.560
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.570 - 1.620
R-factor	0.1962
Rwork	0.194
R-free	0.22170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.011
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.720
High resolution limit [Å]	1.620	1.620
Number of reflections	25346	4028
<I/σ(I)>	10.52
Completeness [%]	99.8
Redundancy	14.47
CC(1/2)	0.988	0.704

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES		293	cell-free crystallization