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8JA4

Structure of the alginate epimerase/lyase

This is a non-PDB format compatible entry.
Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSPRING-8 BEAMLINE BL45XU
Synchrotron siteSPring-8
BeamlineBL45XU
Temperature [K]100
Detector technologyPIXEL
Collection date2022-10-27
DetectorDECTRIS PILATUS 6M
Wavelength(s)1.0000
Spacegroup nameH 3
Unit cell lengths122.520, 122.520, 119.800
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution48.510 - 2.040
R-factor0.1674
Rwork0.167
R-free0.17210
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.015
RMSD bond angle1.638
Data reduction softwareXDS
Data scaling softwareXSCALE
Phasing softwarePHASER
Refinement softwarePHENIX (1.20.1_4487)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]48.5102.110
High resolution limit [Å]2.0402.040
Rmerge0.0591.231
Rmeas0.0621.286
Rpim0.0180.370
Number of reflections426954254
<I/σ(I)>22.191.83
Completeness [%]100.0100
Redundancy1212.1
CC(1/2)1.0000.803
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP2930.1M sodium acetate, pH4.5-pH4.9, 24-30% w/v PEG 4000, 15% v/v glycerol

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