8J9T

Crystal Structure of GyraseA N-terminal at 2.43A Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-01
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.8731
Spacegroup name	P 61
Unit cell lengths	154.781, 154.781, 108.258
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.930 - 2.428
Rwork	0.199
R-free	0.23480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.290
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0411)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	134.040	2.490
High resolution limit [Å]	2.420	2.420
Number of reflections	55446	4093
<I/σ(I)>	8.8
Completeness [%]	99.1
Redundancy	10.1
CC(1/2)	0.993	0.375

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 3500, Tris