8J9R

Crystal structure of UBR box of YIFS-UBR4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-16
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.282
Spacegroup name	P 21 21 21
Unit cell lengths	36.580, 38.280, 55.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.700 - 1.650
R-factor	0.2368
Rwork	0.234
R-free	0.28710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AlphaFold
RMSD bond length	0.008
RMSD bond angle	0.994
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.700	1.709
High resolution limit [Å]	1.650	1.650
Number of reflections	9760	943
<I/σ(I)>	24.84
Completeness [%]	99.3
Redundancy	12.3
CC(1/2)	0.998	0.976

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	0.1 M Bis-Tris (pH5.6), 29% (w/v) polyethylene glycol 3350 (PEG3350), 0.2 M lithium sulfate monohydrate