8J9Q

Crystal structure of UBR box of UBR4 apo

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.283
Spacegroup name	P 21 21 21
Unit cell lengths	43.670, 80.750, 81.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.760 - 2.180
R-factor	0.2217
Rwork	0.219
R-free	0.26880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AlphaFold
RMSD bond length	0.008
RMSD bond angle	0.844
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.760	2.258
High resolution limit [Å]	2.180	2.180
Rmerge	0.150
Number of reflections	15341	1490
<I/σ(I)>	13.99
Completeness [%]	98.0
Redundancy	12.7
CC(1/2)	0.999	0.926

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291.15	0.1 M DL-malic acid (pH7.0) and 18% (w/v) polyethylene glycol 3350 (PEG3350)