8J9F
Structure of STG-hydrolyzing beta-glucosidase 1 (PSTG1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2021-06-22 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 65.300, 113.250, 158.660 |
Unit cell angles | 71.47, 82.68, 79.83 |
Refinement procedure
Resolution | 47.600 - 2.850 |
Rwork | 0.232 |
R-free | 0.28170 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.648 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP (11.9.02) |
Refinement software | REFMAC (5.8.0405) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.020 |
High resolution limit [Å] | 2.850 | 2.850 |
Rmeas | 0.619 | 1.661 |
Number of reflections | 832650 | 131530 |
<I/σ(I)> | 3.65 | 1.33 |
Completeness [%] | 99.6 | 99.8 |
Redundancy | 8.46 | 8.25 |
CC(1/2) | 0.879 | 0.515 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | PEG 3000, imidazole, lithium sulfate |