8J9B

LnaB-actin binary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-01-08
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.979183
Spacegroup name	P 31
Unit cell lengths	110.577, 110.577, 407.927
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	62.100 - 3.420
Rwork	0.287
R-free	0.29800
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	135.000	3.610
High resolution limit [Å]	3.420	3.420
Rmerge	0.200	0.637
Rmeas	0.230	0.733
Rpim	0.111	0.358
Total number of observations		45713
Number of reflections	74997	10997
<I/σ(I)>	7.7	3
Completeness [%]	99.6
Redundancy	4.2	4.2
CC(1/2)	0.975	0.470

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		289	PEG4000,Sodium HEPES,Ammonnium sulfate