8J8Q
Structure of the four-component Paf1 complex from Saccharomyces eubayanus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2019-10-14 |
| Detector | RAYONIX MX170-HS |
| Wavelength(s) | 0.97930 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.634, 89.173, 128.072 |
| Unit cell angles | 90.00, 97.08, 90.00 |
Refinement procedure
| Resolution | 27.070 - 3.110 |
| R-factor | 0.239 |
| Rwork | 0.237 |
| R-free | 0.27800 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.136 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.150 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.157 | 1.116 |
| Number of reflections | 28686 | 1421 |
| <I/σ(I)> | 12.9 | |
| Completeness [%] | 99.5 | |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M Ammonium citrate tribasic pH 7.0; 25% w/v Polyethylene glyco 3,350 |
