8J8P

Structure of the four-component Paf1 complex from Saccharomyces eubayanus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	80
Detector technology	CCD
Collection date	2019-06-30
Detector	RAYONIX MX300-HS
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	71.775, 88.480, 127.775
Unit cell angles	90.00, 97.27, 90.00

Refinement procedure

Resolution	34.470 - 2.700
R-factor	0.227
Rwork	0.225
R-free	0.26250
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.028
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.750
High resolution limit [Å]	2.700	2.700
Rmerge	0.068
Number of reflections	43763	2197
<I/σ(I)>	22.1
Completeness [%]	99.9
Redundancy	3.7
CC(1/2)		0.706

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M Ammonium citrate tribasic pH 7.0; 25% w/v Polyethylene glyco 3,350