8J6V

Structure of yeast Arginyl-tRNA-protein transferase 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 43
Unit cell lengths	235.660, 235.660, 171.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.565 - 3.400
R-factor	0.2401
Rwork	0.238
R-free	0.26990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.696
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.570	48.570	3.490
High resolution limit [Å]	3.400	15.200	3.400
Rmerge	0.371	0.066	2.305
Rmeas	0.386	0.070	2.397
Number of reflections	128824	1464	9478
<I/σ(I)>	9.41
Completeness [%]	99.8
Redundancy	13.7
CC(1/2)	0.991	0.998	0.521

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.5 M ammonium sulfate, 3 % isopropanol