8J68

Crystal structure of the LOV1 R60K mutant of Klebsormidium nitens phototropin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-30
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.965459
Spacegroup name	P 43 21 2
Unit cell lengths	53.905, 53.905, 137.408
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.146 - 1.845
Rwork	0.171
R-free	0.22410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.592
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0403)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.905	5.005
High resolution limit [Å]	1.845	1.845
Number of reflections	15532	965
<I/σ(I)>	12.7
Completeness [%]	84.8
Redundancy	6.5
CC(1/2)	0.999	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	sodium phosphate monobasic monohydrate, potassium phosphate dibasic at pH 6.9