8J68
Crystal structure of the LOV1 R60K mutant of Klebsormidium nitens phototropin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-1 |
Synchrotron site | ESRF |
Beamline | MASSIF-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-09-30 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.965459 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 53.905, 53.905, 137.408 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.146 - 1.845 |
Rwork | 0.171 |
R-free | 0.22410 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.592 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.905 | 5.005 |
High resolution limit [Å] | 1.845 | 1.845 |
Number of reflections | 15532 | 965 |
<I/σ(I)> | 12.7 | |
Completeness [%] | 84.8 | |
Redundancy | 6.5 | |
CC(1/2) | 0.999 | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | sodium phosphate monobasic monohydrate, potassium phosphate dibasic at pH 6.9 |