8J67

Crystal structure of Toxoplasma gondii M2AP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97
Spacegroup name	C 2 2 21
Unit cell lengths	82.588, 183.389, 84.927
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.850 - 1.810
R-factor	0.1834
Rwork	0.182
R-free	0.21510
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.195
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.850	1.840
High resolution limit [Å]	1.810	1.810
Rmerge	0.049	1.340
Number of reflections	59267	3430
<I/σ(I)>	17.4	1.2
Completeness [%]	99.9	98.9
Redundancy	6.8	6.9
CC(1/2)	1.000	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1 M Hepes sodium pH 6.5, 1.35 M sodium citrate tribasic dihydrate