8J66
Crystal structure of glycosyltransferase SgUGT94-289-3 in complex with M3, state 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-04-14 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.068, 78.127, 94.653 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.330 - 2.270 |
R-factor | 0.2081 |
Rwork | 0.207 |
R-free | 0.23410 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.382 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.330 | 2.356 |
High resolution limit [Å] | 2.270 | 2.275 |
Number of reflections | 24762 | 2392 |
<I/σ(I)> | 11.23 | |
Completeness [%] | 98.1 | |
Redundancy | 4.7 | |
CC(1/2) | 0.986 | 0.504 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | 0.2 M Sodium iodide, 20% w/v Polyethylene glycol 3,350, pH 7.0 |