8J64
Crystal structure of Toxoplasma gondii MIC2-M2AP complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-12-27 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.777, 53.176, 95.548 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.030 - 2.000 |
R-factor | 0.177 |
Rwork | 0.175 |
R-free | 0.21140 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.519 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.030 | 2.150 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.057 | 0.084 |
Number of reflections | 14640 | 838 |
<I/σ(I)> | 27.2 | 16.2 |
Completeness [%] | 95.6 | 76.1 |
Redundancy | 8.8 | 6.3 |
CC(1/2) | 0.998 | 0.993 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 15% glycerol, 0.2M sodium acetate trihydrate, 0.1M TRIS hydrochloride pH 8.5, 30% w/v polyethylene glycol 4000 |