8J5J
The crystal structure of bat coronavirus RsYN04 RBD bound to the antibody S43
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979183 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 68.373, 68.373, 240.863 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.550 - 3.000 |
| R-factor | 0.2275 |
| Rwork | 0.227 |
| R-free | 0.23060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.766 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.000 | 3.000 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.123 | 1.819 |
| Number of reflections | 21699 | 2255 |
| <I/σ(I)> | 20.3 | |
| Completeness [%] | 99.8 | |
| Redundancy | 13.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M Potassium nitrate, 20% w/v Polyethylene glycol 3,350 |
