8J5F
Crystal structure of kinase AbmG in complex with cytidine and ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-07 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 71.970, 78.986, 83.332 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.840 - 1.700 |
| R-factor | 0.1545 |
| Rwork | 0.151 |
| R-free | 0.19500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.973 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.840 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.130 | 0.968 |
| Number of reflections | 26511 | 1403 |
| <I/σ(I)> | 15.3 | 2.4 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 14.7 | 10.9 |
| CC(1/2) | 0.998 | 0.854 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.03 M citrate, 0.07 M Bis-Tris propane, 28% PEG 3350 |
