8J5E
Crystal structure of kinase AbmG in complex with 4'-thiocytidine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-07 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.10 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 70.642, 78.108, 84.089 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.470 - 1.700 |
| R-factor | 0.1743 |
| Rwork | 0.171 |
| R-free | 0.21390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.981 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.470 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.108 | 0.682 |
| Number of reflections | 25964 | 1332 |
| <I/σ(I)> | 16.9 | 3.3 |
| Completeness [%] | 99.9 | 99.1 |
| Redundancy | 14.9 | 11.1 |
| CC(1/2) | 0.999 | 0.871 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M magnesium nitrate, 28% PEG3350 |
