8J5B

Crystal structure of P domain from norovirus GI.4 capsid protein.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	93
Detector technology	CCD
Collection date	2016-12-20
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0000
Spacegroup name	P 1
Unit cell lengths	61.673, 62.791, 92.756
Unit cell angles	91.74, 97.71, 119.07

Refinement procedure

Resolution	49.800 - 1.890
R-factor	0.2342
Rwork	0.232
R-free	0.27540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.549
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.800	2.010
High resolution limit [Å]	1.890	1.890
Rmeas	0.128
Rpim		0.731
Number of reflections	90970	14442
<I/σ(I)>	6.98	1.54
Completeness [%]	95.9	94.2
Redundancy	2.31	2.29
CC(1/2)	0.990	0.849

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293.15	0.1M tri-Sodium citrate (pH5.6), 20%(v/v) 2-Propanol , 20%(w/v) Polyethylene glycol 4,000