8J51
Crystal structure of Flavihumibacter petaseus GH31 alpha-galactosidase in complex with galactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-06-25 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 71.357, 72.371, 80.884 |
| Unit cell angles | 101.82, 105.11, 103.67 |
Refinement procedure
| Resolution | 43.695 - 2.150 |
| Rwork | 0.152 |
| R-free | 0.22240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.439 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0405) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.010 | 2.190 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.097 | 0.487 |
| Rmeas | 0.138 | 0.689 |
| Rpim | 0.487 | |
| Number of reflections | 77597 | 4574 |
| <I/σ(I)> | 9.9 | 2.1 |
| Completeness [%] | 98.0 | 97.1 |
| Redundancy | 3.5 | 3.5 |
| CC(1/2) | 0.993 | 0.739 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 1 M sodium chloride, 50 mM MES-NaOH, pH 6.0 |
