8J4B

Crystal structure of OY phytoplasma SAP05 in complex with AtSPL13

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2022-11-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.2828
Spacegroup name	P 21 21 2
Unit cell lengths	90.920, 63.350, 68.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.930 - 2.000
R-factor	0.1771
Rwork	0.175
R-free	0.21310
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	0.926
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.930	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.109
Number of reflections	27306	2608
<I/σ(I)>	13.74	3.21
Completeness [%]	99.5
Redundancy	9.1
CC(1/2)		0.627

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Ammonium acetate, 0.1 M HEPES pH 7.5, 25% w/v Polyethylene glycol 3,350