8J2Y
Acidimicrobiaceae bacterium photocobilins protein, dark state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-09 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 108.773, 125.836, 124.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.310 - 2.300 |
| R-factor | 0.21085 |
| Rwork | 0.209 |
| R-free | 0.23664 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.768 |
| Data reduction software | DIALS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.310 | 2.382 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.177 | 0.944 |
| Number of reflections | 38287 | 3775 |
| <I/σ(I)> | 6.95 | 0.94 |
| Completeness [%] | 99.9 | 99.79 |
| Redundancy | 13.7 | 13.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 8% PEG 20,000, 8% PEG 550 MME, 0.1M Sodium acetate pH5.5, 0.2M Potassium thiocyanate |
