8J2X
Saccharothrix syringae photocobilins protein, light state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-07-08 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 109.667, 109.667, 98.074 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 94.970 - 1.980 |
| R-factor | 0.18394 |
| Rwork | 0.183 |
| R-free | 0.20029 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.551 |
| Data reduction software | DIALS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 98.070 | 2.010 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.072 | 2.404 |
| Rmeas | 0.074 | |
| Number of reflections | 47811 | 4716 |
| <I/σ(I)> | 20.64 | 0.67 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 20.6 | |
| CC(1/2) | 1.000 | 0.600 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2M Potassium citrate tribasic monohydrate 20% w/v PEG 3350 |
