8J2V

Glucosyl transferase NbUGT72AY1 co-crystallized with Scopoletin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.987
Spacegroup name	P 21 21 2
Unit cell lengths	83.580, 103.660, 54.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.890 - 2.000
R-factor	0.1955
Rwork	0.194
R-free	0.22830
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond angle	6.572
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.940	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	31504	2558
<I/σ(I)>	19.02
Completeness [%]	96.9
Redundancy	10.6
CC(1/2)	0.999	0.621

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.2 M Ammonium sulfate, 0.1 M MES pH 6.5, 30 % w/v PEG 5000 MME