8J2L

Crystal structure of a bright green fluorescent protein (StayGold) with double mutations (N137A, Y187F) in jellyfish Cytaeis uchidae from Biortus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 61
Unit cell lengths	135.086, 135.086, 58.427
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.770 - 1.700
Rwork	0.146
R-free	0.16940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AlphaFold
RMSD bond length	0.013
RMSD bond angle	1.811
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.770	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.096	0.855
Number of reflections	67020	3587
<I/σ(I)>	12.8
Completeness [%]	99.9
Redundancy	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M HEPES pH7.5, 1.4 M Na3citrate