8J10

Crystal structure of horse spleen L-ferritin at -180deg Celsius cooled from -20deg Celsius.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E DW
Temperature [K]	93.15
Detector technology	PIXEL
Collection date	2022-11-08
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.542
Spacegroup name	F 4 3 2
Unit cell lengths	180.786, 180.786, 180.786
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.096 - 1.600
Rwork	0.180
R-free	0.20580
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.563
Data reduction software	CrysalisPro (v42)
Data scaling software	Aimless (0.7.9)
Phasing software	MOLREP (11.9.02)
Refinement software	REFMAC (5.8.0403)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.096	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.112	0.955
Rmeas	0.126	1.162
Rpim	0.056	0.649
Number of reflections	33540	1686
<I/σ(I)>	8.8	1.1
Completeness [%]	99.1
Redundancy	4.7
CC(1/2)	0.995	0.530

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293.15	Ammonium sulfate (0.5-1.0M), Cadmium sulfate(12-20mM)