8J0Y
Crystal structure of horse spleen L-ferritin at 20deg Celsius.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E DW |
| Temperature [K] | 293.15 |
| Detector technology | PIXEL |
| Collection date | 2022-11-04 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.5420 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 183.529, 183.529, 183.529 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.432 - 1.600 |
| Rwork | 0.169 |
| R-free | 0.19010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.588 |
| Data reduction software | CrysalisPro (v42) |
| Data scaling software | Aimless (0.7.9) |
| Phasing software | MOLREP (11.9.02) |
| Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.432 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.118 | 1.850 |
| Rmeas | 0.132 | 2.234 |
| Rpim | 0.057 | 1.232 |
| Number of reflections | 35274 | 1740 |
| <I/σ(I)> | 6.9 | 0.5 |
| Completeness [%] | 99.7 | 97.5 |
| Redundancy | 4.7 | 3.1 |
| CC(1/2) | 0.996 | 0.263 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293.15 | Ammonium sulfate (0.5-1.0M), Cadmium sulfate(12-20mM) |
