8J0V

Crystal structure of horse spleen L-ferritin at -100deg Celsius.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E DW
Temperature [K]	173.15
Detector technology	PIXEL
Collection date	2022-12-19
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.5420
Spacegroup name	F 4 3 2
Unit cell lengths	180.624, 180.624, 180.624
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.526 - 1.600
Rwork	0.171
R-free	0.19050
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.622
Data reduction software	CrysalisPro (v42)
Data scaling software	Aimless (0.7.9)
Phasing software	MOLREP (11.9.02)
Refinement software	REFMAC (5.8.0403)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.526	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.099	1.020
Rmeas	0.109	1.203
Rpim	0.046	0.628
Number of reflections	34683	1621
<I/σ(I)>	10.6	1.2
Completeness [%]	99.7
Redundancy	5.2
CC(1/2)	0.997	0.508

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293.15	Ammonium sulfate (0.5-1.0M), Cadmium sulfate(12-20mM)