8J0Q

Structure of DNA binding domain of human TFAP2B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-10
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	51.212, 81.829, 115.628
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.360 - 2.400
R-factor	0.18681
Rwork	0.184
R-free	0.24112
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.605
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.410	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	19146	2025
<I/σ(I)>	12.9
Completeness [%]	97.2
Redundancy	6.5
CC(1/2)	0.995	0.970

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M Sodium citrate tribasic dihydrate and 20% PEG 3350 (w/v)