8J0P

Chitin binding SusD-like protein AqSusD from a marine Bacteroidetes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-02
Detector	DECTRIS PILATUS3 R 200K-A
Wavelength(s)	1.54184
Spacegroup name	C 1 2 1
Unit cell lengths	88.668, 64.227, 105.223
Unit cell angles	90.00, 113.38, 90.00

Refinement procedure

Resolution	19.010 - 2.400
R-factor	0.162
Rwork	0.160
R-free	0.20200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond angle	5.857
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20_4459: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.010	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	21313	2225
<I/σ(I)>	9.5
Completeness [%]	99.6
Redundancy	5.1
CC(1/2)	0.984	0.771

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	0.2 M magnesium chloride, 0.1 M Tris-HCl pH8.5, and 20% (w/v) PEG8000