8J0L

Structure of DNA binding Domain of Human TFAP2A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	50.613, 81.907, 115.939
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.320 - 1.980
R-factor	0.18441
Rwork	0.183
R-free	0.21905
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.616
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.900	2.080
High resolution limit [Å]	1.970	1.970
Number of reflections	34109	4928
<I/σ(I)>	15.9
Completeness [%]	98.6
Redundancy	4.7
CC(1/2)	0.998	0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M Sodium tartrate dibasic dihydrate, 20% PEG 3350 (w/v)