8J0H

Crystal structure of the fission yeast Rex1BD protein(C4H3.06)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	P 21 21 2
Unit cell lengths	159.014, 138.481, 50.897
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.474 - 3.383
R-factor	0.2471
Rwork	0.242
R-free	0.29610
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.469
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASES
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.590
High resolution limit [Å]	3.380	9.970	3.390
Rmerge	0.129	0.027	0.652
Rmeas	0.139	0.029	0.703
Number of reflections	30157	1168	4792
<I/σ(I)>	13.43
Completeness [%]	99.8
Redundancy	3.5
CC(1/2)	0.998	1.000	0.876

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	289	8%v/v Tacsimate pH5.0, 20%w/v polyethylene glycol 3350