8IZS

Structure of a triple-helix region of human collagen type XVII

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2023-04-06
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 1
Unit cell lengths	27.380, 30.050, 40.120
Unit cell angles	78.40, 78.12, 64.27

Refinement procedure

Resolution	38.940 - 1.530
R-factor	0.2073
Rwork	0.206
R-free	0.24190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.141
Data reduction software	iMOSFLM
Data scaling software	pointless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.940	1.585
High resolution limit [Å]	1.530	1.530
Rmerge		0.460
Number of reflections	13538	450
<I/σ(I)>	5.19
Completeness [%]	80.1
Redundancy	3.9
CC(1/2)	0.986	0.841

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.2M Sodium Acetate, 20%(w/v)PEG 3350