8IYT

Crystal Structure of Serine Palmitoyltransferase complexed with D-methylserine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	61.843, 61.843, 208.344
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.185 - 1.700
Rwork	0.173
R-free	0.21860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3a2b
RMSD bond length	0.011
RMSD bond angle	1.706
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.800
High resolution limit [Å]	1.700	1.700
Number of reflections	44526	7144
<I/σ(I)>	20.5
Completeness [%]	97.5
Redundancy	10.1
CC(1/2)	0.999	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG4000, Sodium acetate, Tris-HCl