8IYT
Crystal Structure of Serine Palmitoyltransferase complexed with D-methylserine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-20 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.98 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 61.843, 61.843, 208.344 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.185 - 1.700 |
Rwork | 0.173 |
R-free | 0.21860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3a2b |
RMSD bond length | 0.011 |
RMSD bond angle | 1.706 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.800 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 44526 | 7144 |
<I/σ(I)> | 20.5 | |
Completeness [%] | 97.5 | |
Redundancy | 10.1 | |
CC(1/2) | 0.999 | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG4000, Sodium acetate, Tris-HCl |