8IYI
Spermidine synthase from Kluyveromyces lactis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-07-12 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.252, 98.180, 102.134 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.980 - 1.900 |
R-factor | 0.1692 |
Rwork | 0.168 |
R-free | 0.19870 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.818 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 52396 | 5161 |
<I/σ(I)> | 33 | |
Completeness [%] | 100.0 | |
Redundancy | 12.9 | |
CC(1/2) | 0.996 | 0.965 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 9.2% v/v TacsimateTM pH 5.0, 16.5% w/v PEG 3350 |