8IYE

Structure of VldE-D158N in complex with GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2017-08-23
Detector	RAYONIX MX300-HS
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	48.044, 84.567, 120.928
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.040 - 1.900
R-factor	0.238
Rwork	0.236
R-free	0.28310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.685
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.040	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.094	0.544
Number of reflections	74663	4474
<I/σ(I)>	6.7	1.7
Completeness [%]	97.9
Redundancy	4.1
CC(1/2)	0.996	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM Tris-HCl, pH8.5, 26% PEG 4000