8IXW
Tilapia lake virus S8 segment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 298.15 |
Detector technology | CCD |
Collection date | 2020-12-25 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9791 |
Spacegroup name | P 1 |
Unit cell lengths | 50.486, 51.342, 52.706 |
Unit cell angles | 106.86, 108.92, 108.02 |
Refinement procedure
Resolution | 20.960 - 1.800 |
R-factor | 0.1776 |
Rwork | 0.176 |
R-free | 0.20320 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.865 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.19.2_4158) |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.960 | 1.864 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.039 | 0.211 |
Rmeas | 0.046 | 0.247 |
Rpim | 0.024 | 0.127 |
Number of reflections | 38281 | 3781 |
<I/σ(I)> | 20.12 | |
Completeness [%] | 96.2 | |
Redundancy | 3.6 | |
CC(1/2) | 0.998 | 0.982 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 289.15 | 0.08 M Potassium chloride, 0.02 M Barium chloride dihydrate, 0.04 M Sodium cacodylate trihydrate pH 6.0, 40% v/v (+/-)-2-Methyl-2,4-pentanediol, 0.012 M Spermine tetrahydrochloride. |