8IX6

Crystal structure of Pyruvic Oxime Dioxygenase (POD) from Bradyrhizobium sp. WSM3983

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2017-12-15
Detector	DECTRIS EIGER X 4M
Wavelength(s)	2.7
Spacegroup name	I 4 3 2
Unit cell lengths	153.896, 153.896, 153.896
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	76.950 - 2.470
R-factor	0.2314
Rwork	0.230
R-free	0.25770
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.514
Data scaling software	XDS
Phasing software	CRANK2
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.950	2.510
High resolution limit [Å]	2.470	2.470
Rmerge	0.087	1.766
Number of reflections	11546	569
<I/σ(I)>	43.9
Completeness [%]	100.0
Redundancy	38.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	1.56M ammonium sulfate, 25% (v/v) glycerol