8IWG
Pseudomoans Aerugiona Wildtype Ketopantoate Reductase native structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER X8 PROTEUM |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-01 |
| Detector | Bruker PHOTON III |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.098, 47.691, 96.271 |
| Unit cell angles | 90.00, 99.31, 90.00 |
Refinement procedure
| Resolution | 28.704 - 2.150 |
| R-factor | 0.1967 |
| Rwork | 0.195 |
| R-free | 0.22780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.682 |
| Data reduction software | PROTEUM PLUS |
| Data scaling software | PROTEUM PLUS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.710 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 33097 | 3276 |
| <I/σ(I)> | 8.6 | |
| Completeness [%] | 100.0 | |
| Redundancy | 10.4 | |
| CC(1/2) | 0.990 | 0.778 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 295 | H |
