8IWC
Crystal structure of Q9PR55 at pH 6.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2022-12-16 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.433, 54.144, 77.184 |
| Unit cell angles | 90.00, 92.06, 90.00 |
Refinement procedure
| Resolution | 28.200 - 2.430 |
| R-factor | 0.1859 |
| Rwork | 0.184 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.563 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.200 | 2.517 |
| High resolution limit [Å] | 2.430 | 2.430 |
| Rmerge | 0.080 | 0.704 |
| Rmeas | 0.086 | 0.761 |
| Rpim | 0.031 | 0.286 |
| Number of reflections | 23390 | 2280 |
| <I/σ(I)> | 23.9 | 2.1 |
| Completeness [%] | 99.8 | 99 |
| Redundancy | 7.4 | 6.5 |
| CC(1/2) | 0.900 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | MES, sodium potassium tartrate |
