8IWB
Crystal structure of Q9PR55 at pH 7.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2022-12-16 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.404, 54.156, 76.664 |
Unit cell angles | 90.00, 92.28, 90.00 |
Refinement procedure
Resolution | 28.780 - 2.420 |
R-factor | 0.2403 |
Rwork | 0.238 |
R-free | 0.28220 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.915 |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.780 | 2.511 |
High resolution limit [Å] | 2.420 | 2.425 |
Rmerge | 0.084 | 0.742 |
Rmeas | 0.090 | 0.796 |
Rpim | 0.033 | 0.286 |
Number of reflections | 22464 | 2318 |
<I/σ(I)> | 19.3 | 2 |
Completeness [%] | 95.9 | 100 |
Redundancy | 7.5 | 7.6 |
CC(1/2) | 0.903 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | sodium HEPES, sodium dihydrogen phosphate, sodium dihydrogen phosphate |