8IW5

Crystal structure of liprin-beta H2H3 dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	29.793, 50.637, 30.780
Unit cell angles	90.00, 113.44, 90.00

Refinement procedure

Resolution	27.330 - 1.700
R-factor	0.2236
Rwork	0.221
R-free	0.27170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.774
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.730
High resolution limit [Å]	1.700	4.610	1.700
Rmerge	0.104	0.076	0.724
Rmeas	0.116	0.087	0.832
Rpim	0.051	0.041	0.396
Total number of observations	49910
Number of reflections	8724	349	431
<I/σ(I)>	8.4
Completeness [%]	93.3	71.5	92.7
Redundancy	5.7	4.3	4.3
CC(1/2)	0.992	0.990	0.704

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.05 M Calcium acetate, 0.1 M Sodium cacodylate pH 6.0 and 25% v/v MPD