8IVJ
Candida boidinii Formate Dehydrogenase V120T Mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU PhotonJet-R |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-03-16 |
Detector | RIGAKU HyPix-3000 |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 |
Unit cell lengths | 53.664, 68.611, 109.430 |
Unit cell angles | 77.96, 89.63, 81.40 |
Refinement procedure
Resolution | 24.300 - 1.900 |
R-factor | 0.2474 |
Rwork | 0.247 |
R-free | 0.27120 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.568 |
Data reduction software | CrysalisPro |
Data scaling software | CrysalisPro |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 106.990 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rpim | 0.195 | |
Number of reflections | 118611 | 7897 |
<I/σ(I)> | 3 | |
Completeness [%] | 99.7 | 99.5 |
Redundancy | 7.3 | 4.6 |
CC(1/2) | 0.535 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 30% (w/v) PEG 5000 MME, 100 mM MES/Sodium hydroxide pH 6.5, 200 mM Ammonium sulfate |