8IVJ
Candida boidinii Formate Dehydrogenase V120T Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU PhotonJet-R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-16 |
| Detector | RIGAKU HyPix-3000 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.664, 68.611, 109.430 |
| Unit cell angles | 77.96, 89.63, 81.40 |
Refinement procedure
| Resolution | 24.300 - 1.900 |
| R-factor | 0.2474 |
| Rwork | 0.247 |
| R-free | 0.27120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.568 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 106.990 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rpim | 0.195 | |
| Number of reflections | 118611 | 7897 |
| <I/σ(I)> | 3 | |
| Completeness [%] | 99.7 | 99.5 |
| Redundancy | 7.3 | 4.6 |
| CC(1/2) | 0.535 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 30% (w/v) PEG 5000 MME, 100 mM MES/Sodium hydroxide pH 6.5, 200 mM Ammonium sulfate |
