8IVF
FABP7 complexed with 25-HC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2019-03-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.700, 54.110, 67.240 |
| Unit cell angles | 90.00, 92.34, 90.00 |
Refinement procedure
| Resolution | 42.140 - 2.600 |
| R-factor | 0.2151 |
| Rwork | 0.211 |
| R-free | 0.24860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.027 |
| RMSD bond angle | 1.492 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.140 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.043 | 0.043 |
| Number of reflections | 13896 | 748 |
| <I/σ(I)> | 58.48 | |
| Completeness [%] | 92.9 | |
| Redundancy | 19.18 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 0.1 M Bis-Tris pH 5.5 25% w/v PEG 3350 |
