8IUI
Crystal structure of silkworm fibroinase complexed with E-64
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-18 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.180, 64.690, 92.910 |
| Unit cell angles | 90.00, 97.64, 90.00 |
Refinement procedure
| Resolution | 92.085 - 1.560 |
| Rwork | 0.231 |
| R-free | 0.26930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.601 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.090 | 1.600 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Rmerge | 0.131 | 0.392 |
| Rmeas | 0.143 | 0.425 |
| Rpim | 0.055 | 0.164 |
| Number of reflections | 58638 | 4333 |
| <I/σ(I)> | 8.1 | |
| Completeness [%] | 99.6 | 98.8 |
| Redundancy | 6.7 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 30% w/v Polyethylene glycol monomethyl ether 2,000, 0.1 M Potassium thiocyanate |
