8IUC

Crystal structure of GH65 alpha-1,2-glucosidase from Flavobacterium johnsoniae in complex with isomaltose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-05
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	122.938, 194.090, 111.880
Unit cell angles	90.00, 116.35, 90.00

Refinement procedure

Resolution	44.710 - 1.560
Rwork	0.187
R-free	0.21140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.974
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0405)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.540	1.590
High resolution limit [Å]	1.560	1.560
Rmerge	0.072	0.947
Rmeas	0.088	1.150
Rpim	0.049	0.644
Number of reflections	326516	326516
<I/σ(I)>	13.2	2
Completeness [%]	98.2
Redundancy	6.1
CC(1/2)	0.999	0.813

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	300mM ammonium citrate, pH 7.0-8.0, 10mM TCEP, 12% PEG 3350