8IU8
Crystal structure of GH66 endodextranase from Flavobacterium johnsoniae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-03-05 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 156.241, 48.409, 76.453 |
Unit cell angles | 90.00, 104.60, 90.00 |
Refinement procedure
Resolution | 47.300 - 1.850 |
Rwork | 0.175 |
R-free | 0.23040 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 2.151 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0405) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.300 | 1.890 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.112 | 0.875 |
Rmeas | 0.134 | 1.033 |
Rpim | 0.073 | 0.546 |
Number of reflections | 47439 | 47439 |
<I/σ(I)> | 10.1 | 1.9 |
Completeness [%] | 99.7 | |
Redundancy | 6.3 | |
CC(1/2) | 0.995 | 0.870 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100mM Tris-HCl, pH 8.5-9.0, 200mM lithium sulfate, 20% PEG 4000 |