8IU6
Crystal structure of peptidyl-tRNA hydrolase mutant from Enterococcus faecium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2023-03-01 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54178 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 130.739, 49.435, 65.500 |
Unit cell angles | 90.00, 107.87, 90.00 |
Refinement procedure
Resolution | 32.110 - 2.900 |
Rwork | 0.235 |
R-free | 0.28600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7y52 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.881 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.340 | 3.010 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 9021 | 861 |
<I/σ(I)> | 21.7 | |
Completeness [%] | 93.0 | |
Redundancy | 2.1 | |
CC(1/2) | 0.980 | 0.950 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 0.1M Tris-HCl pH 7.5 0.2M Ammonium Sulfate 25% PEG 4K |