8ITN
Crystal structure of USP47apo catalytic domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2013-08-02 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 94.077, 58.908, 251.932 |
| Unit cell angles | 90.00, 96.69, 90.00 |
Refinement procedure
| Resolution | 35.500 - 2.600 |
| R-factor | 0.2586 |
| Rwork | 0.258 |
| R-free | 0.26940 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.503 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 6.500 | 2.690 |
| High resolution limit [Å] | 2.600 | 5.600 | 2.600 |
| Rmerge | 0.073 | 0.032 | 0.291 |
| Total number of observations | 223996 | ||
| Number of reflections | 41388 | 8250 | 7584 |
| <I/σ(I)> | 9.4 | ||
| Completeness [%] | 93.3 | 99.3 | 90.1 |
| Redundancy | 2.9 | 3.6 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 0.2 M imidazole-malate (pH 5.5) and 18% (v/v) PEG 4000 |
